Protein engineering is the process of changing protein structures and assign them with new and/or desirable qualities in terms of activity, solubility, affinity, specificity, stability, resistance, and so on. De novo design, rational design, and direct evolution are the protein engineering methodologies that are most frequently used.
With the rapid growth of genome sequencing data, an increasing number of proteins have emerged with great research interest. Many efforts have been made toward the development of various methodologies that have proven efficient in the biophysical characterization of biomolecules in order to better understand their biological activities and pave the way for structure-function relationship investigations.
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